The respiratory explosion activity of neutrophils in blood from patients with Graves' disease manifestation

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Abstract


 

Objective. To study respiratory burst activity in neutrophilic granulocytes in the onset of Graves’ disease (GD).

Material and methods. Twenty-six females aged 18—55 years (mean age, 40.7±13.2 years), with the diagnosis of Graves’ disease verified for the first time, were enrolled in this study. Spontaneous and zymosan-induced chemiluminescence (CL) was assessed using a CL3606 36-channel analyzer for 90 min. The following parameters of the chemiluminescence curve were determined: time to attain the maximum (Tmax), the maximum intensity (Imax), and the area under the curve (S).

Results. Patients with GD and lucigenin-dependent CL of neutrophils exhibited decreased zymosan-induced CL and the increased Imax of luminol-dependent spontaneous and zymosan-induced CL of neutrophils. Association between the anti-TPO level in blood serum with Tmax (r=–0,70; р=0.036) and Imax of luminol-dependent induced CL (r=–0.72; р=0.030) was found. The maximum level of synthesis of secondary reactive oxygen species (ROS) was found to be elevated in patients with GP both at relative rest and upon antigen-induced respiratory burst.

Conclusion. In patients with GD, respiratory burst activity in neutrophils increases due to synthesis of secondary reactive oxygen species. Upon the onset of GD, already at the stage of subclinical thyrotoxicosis, the association between the indices of respiratory burst in neutrophils and thyroid hormones is lost but there emerges a relationship between the CL response and anti-TPO concentration.


Sergey A. Dogadin

sadogadin@gmail.com
ORCID iD: 0000-0002-1709-466X
SPIN-code: 4803-3756
V.F. Voino-Yasenetsky Krasnoyarsk State Medical University; Regional Clinical Hospital
Russian Federation, 1 P.Zeleznyaka street, Krasnoyarsk, 660022 

MD, Professor of chair internal diseases №2 with postgraduate course , The Head of Krasnoyarsk regional endocrinology center

Margarita A. Dudina

Author for correspondence.
margo85_@bk.ru
ORCID iD: 0000-0002-2776-927X
SPIN-code: 4854-1926
V.F. Voino-Yasenetsky Krasnoyarsk State Medical University; Regional Clinical Hospital
Russian Federation, 1 P.Zeleznyaka street, Krasnoyarsk, 660022 

PhD, assistent of  internal diseases №2  chair  with postgraduate course, endocrinologyst of endocrinology department Krasnoyarsk clinical hospital

Andrey A. Savchenko

aasavchenko@yandex.ru
ORCID iD: 0000-0001-5829-672X
SPIN-code: 3132-8260
V.F. Voino-Yasenetsky Krasnoyarsk State Medical University; Research Institute of Medical Problems of the North
Russian Federation, 1 P.Zeleznyaka street, Krasnoyarsk, 660022

 MD, Professor, The Head of  physiology  department named after Professor A. T. Pshonica the Krasnoyarsk state medical University im. after Professor V. F. Voyno-Yasenetsky, Krasnoyarsk, Russia.The Head of molecular and cell biology and physiology of Scientific Institution «Scientific Research Institute of medical problems of the North» 

Vladimir A. Mankovskiy

manvlad@inbox.ru
ORCID iD: 0000-0002-4328-8054
SPIN-code: 9283-7490
Regional Clinical Hospital
Russian Federation, 3A P.Zeleznyaka street, Krasnoyarsk, 660022 

endocrinologyst surgeon

Ivan I. Gvozdev

leshman-mult@mail.ru
ORCID iD: 0000-0002-1041-9871
SPIN-code: 6203-4651
Research Institute of Medical Problems of the North
Russian Federation, 3G P.Zeleznyaka street, Krasnoyarsk, 660022 

research assistant

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Copyright (c) Dogadin S.A., Dudina M.A., Savchenko A.A., Mankovskiy V.A., Gvozdev I.I.

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