Effect of oxidative stress on major plasma carotenoid content and composition in prepubertal children with growth hormone deficiency

Cover Page
Open Access Open Access
Restricted Access Subscription Access

Abstract


Objective and hypotheses. This study aimed at examining the effect of oxidative stress on amount and composition of major plasma carotenoids in prepubertal children with growth hormone deficiency (GHD).

Material and methods. Thirteen prepubertal treatment-naive children (2 girls, 11 boys; aged 3.5—12.0 yr, median 8.0 years; bone age 1.5—8.0 yr; median 6.0 years,) with GHD and 7 prepubertal health children (7 boys; aged 6—11 years; median 9.3 years) were included in the study. The levels and composition of carotenoids (lutein with zeaxanthin, lycopene isomers, β-cryptoxanthin, β- and α-carotene and ketocarotenoids) were measured using reverse phase HPLC. Activity of the antioxidant system was assayed via thiobarbituric acid reactive substances (TBARS), ceruloplasmin (CP) levels and total antioxidant capacity (TAC) of plasma. Total cholesterol (TC), low density lipoprotein cholesterol (LDL-C), high density lipoprotein cholesterol (HDL-C), and triglycerides (TG) were also measured.

Results. The levels of TBARS, TC and LDL-C in the GHD children were higher than in healthy children (median 5.6 vs 3.8 µM/L, 4.00 vs 4.37 and 2.40 vs 2.70 mM/L, respectively). Total carotenoid level did not significantly differ between control and the GHD groups. However, contents of lutein and β-cryptoxanthin were significantly lower in the GHD children in comparison with control group (21.34 vs 6.97 and 25.23 vs 10.08 mg/l, respectively). In contrast, levels of lycopene, α- and β-carotene did not significantly differ in the GHD and control groups. At the same time, the level of ketocarotenoids in the GHD children increases (35.67 vs 114.9 mg/l).

Conclusions. We observed that the presence of mild oxidative stress leads to a changes in carotenoid profile of GHD children.


Maria S. Pankratova

Author for correspondence.
ms_pankratova@mail.ru
Endocrinology Research Centre, Moscow
Russian Federation, 11 Dm.Ulyanova street, 117036 Moscow

MD, PhD

Alexander I. Yusipovich

sanyavor@gmail.com
Lomonosov Moscow State University
Russian Federation, 1 Leninskie Gory, 119991 Moscow

PhD

Maria V. Vorontsova

maria.vorontsova.endo@gmail.com
Endocrinology Research Centre, Moscow
Russian Federation, 11 Dm.Ulyanova street, 117036 Moscow

MD

Tila T. Knyazeva

shmushkovich_til@mail.ru
Endocrinology Research Centre, Moscow
Russian Federation, 11 Dm.Ulyanova street, 117036 Moscow

MD

Adil A. Baizhumanov

adilbayzhumanov@icloud.com
Lomonosov Moscow State University
Russian Federation, 1 Leninskie Gory, 119991 Moscow

PhD

Tatiana Y. Shiryaeva

tasha-home@list.ru
Endocrinology Research Centre, Moscow
Russian Federation, 11 Dm.Ulyanova street, 117036 Moscow

MD, PhD

Alexey E. Solovchenko

solovchenko@mail.bio.msu.ru
Lomonosov Moscow State University
Russian Federation, 1 Leninskie Gory, 119991 Moscow

PhD

Georgy V. Maksimov

gmaksimov@mail.ru
Lomonosov Moscow State University
Russian Federation, 1 Leninskie Gory, 119991 Moscow

PhD, Professor

Valentina A. Peterkova

peterkovava@hotmail.com
Endocrinology Research Centre, Moscow
Russian Federation, 11 Dm.Ulyanova street, 117036 Moscow

MD, PhD, Professor

  • Carotenoids in Health and Disease. New York: Marcel Dekker. 2004.
  • Khachik F. Distribution and metabolism of dietary carotenoids in humans as a criterion for development of nutritional supplements. Pure Appl Chem. 2006;78(8):1551-1557. doi: 10.1351/pac200678081551.
  • Mohn A, Marzio D, Giannini C, et al. Alterations in the oxidant-antioxidant status in prepubertal children with growth hormone deficiency: effect of growth hormone replacement therapy. Clinical Endocrinology (Oxford). 2005;63(5):537-542. doi: 10.1111/j.1365-2265.2005.02378.x.
  • Pankratova MS, Baizhumanov AA, Yusipovich AI, et al. Imbalance in the blood antioxidant system in growth hormone-deficient children before and after 1 year of recombinant growth hormone therapy. Peer J. 2015;3:e1055. doi: 10.7717/peerj.1055.
  • Pankratova M, Yusipovich AI, Vorontsova MV, et al. Peculiarities of the blood oxygen and antioxidant status in the children presenting with somatotrophic insufficiency and managed by the substitution treatment with growth hormone. Problemy Endokrinologii. 2012;58(5):10-15. doi: 10.14341/probl201258510-15.
  • Akhalaia MI, Baizhumanov AA, Brazhe NA, et al. Modifying effect of nitric oxide on rat blood plasma proteins and hemoglobin. Dokl Biochem Biophys. 2007;415:222-224. doi: 10.1134/S1607672907040151.
  • Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Analytical Biochemistry. 1979;95(2):351-358. doi: 10.1016/0003-2697(79)90738-3.
  • Folch J, Lees M, Stanley GHS. A simple method for the isolation and purification of total lipides from animal tissues. Journal of Biological Chemistry. 1957;226(1):497-509.
  • Solovchenko AE, Selivanova EA, Chekanov KA, et al. Induction of secondary carotenogenesis in new halophile microalgae from the genus Dunaliella (Chlorophyceae). Biochemistry (Moscow). 2015;80(11):1508-1513. doi: 10.1134/s0006297915110139.

Views

Abstract - 340

PDF (Russian) - 10

Remote (Russian) - 6


Copyright (c) 2017 Pankratova M.S., Yusipovich A.I., Vorontsova M.V., Knyazeva T.T., Baizhumanov A.A., Shiryaeva T.Y., Solovchenko A.E., Maksimov G.V., Peterkova V.A.

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.