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Immunoregulatory proteins and cytokines in the blood of patients with Graves’ disease

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Abstract


Background. Immunoregulatory proteins (alpha-2-macroglobulin, lactoferrin) actively participate in inflammatory and autoimmune processes, affect synthesis and transport of hormones and cytokines, and control cell proliferation and apoptosis. However, the role of these proteins in the pathogenesis of Graves’ disease (GD) is poorly understood.

Objective — the study objective was to determine blood levels of alpha-2-macroglobulin (α2-MG), lactoferrin (LF), and cytokines (TNF-α, IL-6, IL-8, and IFN-γ) in GD.

Material and methods. We determined blood levels of TSH, free T4, TSH receptor antibodies, TNF-α, IL-6, IL-8, IFN-γ, and LF by ELISA as well as α2-MG by quantitative rocket immunoelectrophoresis in 50 patients with decompensated and compensated (4―6 months and 1.5―2 years after treatment onset) GD and 25 healthy females (control group).

Results. GD clinically manifested by body weight l in 84% of patients, sinus tachycardia in almost all patients, paroxysmal atrial fibrillation in 18% of cases, endocrine ophthalmopathy in 12% of patients, and neurological changes. In decompensated GD, there was a statistically significant increase in levels of IFN-γ, IL-6, and α2-MG and an especially significant increase in levels of IL-8 and LF. At 4―6 months after treatment onset, clinical manifestations were stopped in all patients, levels of IL-6 and α2-MG decreased, but the concentrations of TSH receptor antibodies (TSHR-Abs), IL-8, IFN-γ, and LF remained elevated. At 1.5―2 years, levels of the studied proteins and cytokines did not differ from those in the control group.

Conclusion. An increase in blood levels of IL-8, LF, IL-6, and α2-MG in incident or recurrent GD and a decrease in the levels during treatment confirm involvement of immunoregulatory proteins in pathogenesis of the disease.


Tatjana P. Maklakova

Novokuznetsk State Institute for Further Training of Physicians — Branch Campus of the Russian Medical Academy of Continuous Professional Education

Email: maclacovatat@yandex.ru
ORCID iD: 0000-0001-8790-9337
SPIN-code: 9314-5582

Russian Federation,  5, Stroiteley ave., Novokuznetsk, 654005

PhD, MD, Professor

Veronika N. Zorina

Novokuznetsk State Institute for Further Training of Physicians — Branch Campus of the Russian Medical Academy of Continuous Professional Education

Author for correspondence.
Email: nilimmun@yandex.ru
ORCID iD: 0000-0001-9183-7663
SPIN-code: 1630-1716

Russian Federation,  5, Stroiteley ave., Novokuznetsk, 654005

PhD, MD

Anna V. Yanysheva

Novokuznetsk State Institute for Further Training of Physicians — Branch Campus of the Russian Medical Academy of Continuous Professional Education

Email: Anna-nik_06@mail.ru
ORCID iD: 0000-0002-6982-9058

Russian Federation,  5, Stroiteley ave., Novokuznetsk, 654005

endocrinologist, clinical resident of the Department of Endocrinology and Diabetology

Ekaterina V. Mezentseva

Novokuznetsk State Institute for Further Training of Physicians — Branch Campus of the Russian Medical Academy of Continuous Professional Education

Email: perinatal_nk@mail.ru
ORCID iD: 0000-0002-5770-1348

Russian Federation,  5, Stroiteley ave., Novokuznetsk, 654005

endocrinologist, clinical resident of the Department of Endocrinology and Diabetology

Nikolay A. Zorin

Novokuznetsk State Institute for Further Training of Physicians — Branch Campus of the Russian Medical Academy of Continuous Professional Education

Email: macroglobulin@yandex.ru
ORCID iD: 0000-0003-4865-5826
SPIN-code: 4223-0819

Russian Federation,  5, Stroiteley ave., Novokuznetsk, 654005

Doctor of Biological Sciences, Professor, Head of the Scientific Laboratory of Immunology

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Copyright (c) 2019 Maklakova T.P., Zorina V.N., Yanysheva A.V., Mezentseva E.V., Zorin N.A.

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